Anti-Hu CD261 Purified TNFRSF10A Monoclonal Antibody

Product Name

Anti-Hu CD261 Purified TNFRSF10A Monoclonal Antibody

View all TRAILR1/TNFRSF10A Antibodies

SKU/Catalog Number

M02152

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD261 Purified TNFRSF10A Monoclonal Antibody (Catalog# M02152). Tested in Flow Cytometry, IP, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD261 Purified TNFRSF10A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02152)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

DR-4-02

Isotype

Mouse IgG1

Immunogen

Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1. The mouse monoclonal antibody DR-4-02 recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M02152 is reactive to TNFRSF10A in Human

Applications

M02152 is guaranteed for Flow Cytometry, IP, ICC Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

50.089kDa

Background of TRAILR1/TNFRSF10A

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of TNFRSF10A using Anti-Hu CD261 Purified TNFRSF10A Monoclonal Antibody (M02152).

Flow cytometry analysis of TRAIL-R1 expression on the surface of hematopoietic cell lines.
Cells were stained with purified anti-TRAIL-R1 antibodies followed by Goat anti-mouse IgG PE.

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Figure 2. Immunocytochemistry staining of TNFRSF10A using Anti-Hu CD261 Purified TNFRSF10A Monoclonal Antibody (M02152).

Immunocytochemistry staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with TRAIL-R1 expression plasmid using anti-human TRAIL-R1 (DR-4-02).

Gene/Protein Information For TNFRSF10A (Source: Uniprot.org, NCBI)

Alternative Names

APO2; CD261 antigen; CD261; cytotoxic TRAIL receptor; Death receptor 4; DR4 TRAIL receptor 1; DR4; TNF-related apoptosis inducing ligand receptor 1; TNF-related apoptosis-inducing ligand receptor 1; TNFRSF10A; TRAIL R1; TRAILR1; TRAIL-R1; TRAILR-1; TRAILR1MGC9365; tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily, member 10a TNFRSF10A APO2, CD261, DR4, TRAILR-1, TRAILR1 TNF receptor superfamily member 10a tumor necrosis factor receptor superfamily member 10A|TNF-related apoptosis-inducing ligand receptor 1|TRAIL receptor 1|TRAIL-R1|cytotoxic TRAIL receptor|death receptor 4|tumor necrosis factor receptor superfamily member 10a variant 2|tumor necrosis factor receptor superfamily, member 10a

For more info on TNFRSF10A, check out the TNFRSF10A Infographic

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In this infographic, you will see the following information for TNFRSF10A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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